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. 2021 Oct 12;16(10):e0258315. doi: 10.1371/journal.pone.0258315

Fig 1. The Nedd4 family of ligases contains conserved WW domains for interactor recognition.

Fig 1

(A) Nedd4 and related ligases contain 2–4 WW domains that recognize interactors containing a PY motif (PPxY, LPxY) or phosphorylated threonine or serine residues. (B) Alignment of the four WW domains from prototypical member Nedd4 shows moderate sequence similarity and highlights conserved residues, including the two characteristic tryptophan residues (indicated by red arrows). (C) Solution state NMR structure of the Nedd4 WW domain 3 (grey) in complex with a PY motif peptide (red) from a known Nedd4 substrate (PDB ID: 2KPZ, unpublished) reveals key residues (blue) involved in peptide binding.