Fig 2. UpSet analysis and Gene Ontology annotation reveal that Nedd4 family ligases are functionally distinct.

(A) Cross-reference of annotated interactors in the BioGrid database reveals that approximately one quarter of all interactors of the Nedd4 family are recognized by 2+ ligases, revealing little overlap in the known interactomes of the Nedd4 family. Data analysis performed with the UpSet plot tool [76] and graphic annotated in Adobe Illustrator. (B) Gene ontology analysis via the PANTHER database [43, 44] reveals that each Nedd4 family ligase interactome has similar trends in biological process (top) but distinct patterns in protein class composition (bottom).