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. 2021 Sep 30;17(9):e1009779. doi: 10.1371/journal.pgen.1009779

Fig 7. Effect of cenh3-4 mutation on the localization of CENH3 on Arabidopsis centromeres.

Fig 7

(A) Immunodetection of CENH3 in PMCs and tapetum cells using α-CENH3 antibody (green); DNA is counterstained with DAPI (blue). Longer exposure times for EMS30, EMS155, and cenh3-4 were applied to detect the signal. Scale bar corresponds to 5 μm. (B) Association of CENH3 with the CEN180 satellite repeat determined by chromatin immunoprecipitation with CENH3 antibody and dot blot hybridization. Antibody against histone H3 was used as a control. (C) Quantification of the CEN180 repeat in the ChIP experiments by qPCR. Error bars represent standard deviation of three technical replicates. Three independent experiments are presented.