Clinical indicators of asthma-associated symptoms and metagenomic reconstruction pipeline. (a) Statistical differences in the clinical parameters between groups were evaluated with Wilcox test or t test. (b) The workflow of sampling, assembling of metagenome-assembled genomes (MAGs), binning, refinement, and dereplication. A total of 389 high-quality species-level genome bins (SGBs) were identified in the complete data set. com and con represent levels of completeness and contamination, respectively.