TABLE 5.
Primers and sequences used in this study
| Primer | Sequence (5′–3′) |
|---|---|
| spoVS1RACE-R | CGCTGGGATACAAATCAGGTCCAAACCACTAGGCGC |
| spoVS2RACE-R | CCACTTGGAGCGACGAACCCTCTTGCAATCGC |
| spoVS1-a | AGATCTATCGATGCATGCCATGGTACCCGGGAGCTTATCTTTGTGAACTGTAATGG |
| spoVS1-b | CCTATCACCTCAAATGGTTCGCTGGTAGAACCTCGTTAAGTTTAAACAAC |
| S1Km-a | GTTGTTTAAACTTAACGAGGTTCTACCAGCGAACCATTTGAGGTGATAGG |
| S1Km-b | CTGCAAACAGGTTGTTTAAACTTAAAATTCCTCGTAGGCGCTCG |
| spoVS1-c | CGAGCGCCTACGAGGAATTTTAAGTTTAAACAACCTGTTTGCAG |
| spoVS1-d | GCGTCTGCAGAAGCTTCTAGAATTCGAGCTCCACGTGGGTGCTCGCAAAGTTTCA |
| spoVS2-a | AGATCTATCGATGCATGCCATGGTACCCGGGAGTTTGAAGAATGATAGTATGAACCCAAA |
| spoVS2-b | CCTCAAATGGTTCGCTGTTCCATGTAAGTTGCTCCCTCTATT |
| S2Km-a | AATAGAGGGAGCAACTTACATGGAACAGCGAACCATTTGAGG |
| S2Km-b | CCCTTATAGTAACGCTCTCTTCTACTAAAATTCCTCGTAGGCGCTCG |
| spoVS2-c | CGAGCGCCTACGAGGAATTTTAGTAGAAGAGAGCGTTACTATAAGGG |
| spoVS2-d | GCGTCTGCAGAAGCTTCTAGAATTCGAGCTCCAAGAATAAAGCTCTCATTCAACGC |
| spoVS1-F | GCATGCCTGCAGGTCGACTCTAGAGCCATGCGGAGACTACAAGTGA |
| spoVS1-R | TGTAAAACGACGGCCAGTGAATTCGTTGTTTAAACTTAACGAGGTTCTAC |
| spoVS2-F | GCATGCCTGCAGGTCGACTCTAGAGCGAGTTTGAAGAATGATAGTATGAACCC |
| spoVS2-R | TGTAAAACGACGGCCAGTGAATTCCCCTTATAGTAACGCTCTCTTCTA |
| PspoVS1-F | AACTGCAGCCATGCGGAGACTACAAGTGA |
| PspoVS1-R | AACTGCAGTTCCATTCCGCGTTTCCTCCTCGT |
| PspoVS2-F | AACTGCAGATGAAGATGAATCACAATTGGCG |
| PspoVS2-R | CGGGATCCTTCCATGTAAGTTGCTCCCTCTATT |
| EMspoVS1-F | AGTGCAGTGTTAATTGATGTGG |
| EMspoVS1-R | TTCCATTCCGCGTTTCCTCCTCGT |
| EMspoVS2-F | GCTGTATGGATGTTATCATTTGGTG |
| EMspoVS2-R | TTCCATGTAAGTTGCTCCCTCTATT |
| sigH-F | CGGATCCGGTGGAAGCAGGCTTCGTAAG |
| sigH-R | GTCGACTGAATTTAAAGTTGTACTTTC |
| pMAD-F | GGTACCTACGTAGGATCGATCC |
| pMAD-R | TTGCAGGCCATGCTGTCCA |
| HDspoVS1-F | GTTAGAAACTGCGGTACAAAAGTG |
| HDspoVS1-R | CTTTTTAGAGTGTGGGGCGCAGGC |
| HDspoVS2-F | CGAAACTCTATAATCACCAGCTTG |
| HDspoVS2-R | CACGAGGCCATACTTCCCTACCAG |