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. 2021 Oct 6;9(2):e00262-21. doi: 10.1128/Spectrum.00262-21

FIG 4.

FIG 4

DBGWAS analysis for GAM and TYL resistance in 95 Belgian M. bovis isolates and M. bovis PG45. (A) Association of GAM (non-WT, n = 43) and TYL (non-WT, n = 50) genotypes with phenotypes resulted in a shared 23S rRNA target association. (B) Secondary structure of domain V of both 23S rRNA alleles, showing the observed mutations (A2058 and A2062). The 23S rRNA positions are labeled according to classic E. coli numbering. (C) DBGWAS analysis output highlights a complex k-mer web, including continuous k-mer strands, suggesting the association of a genetic operon with the phenotype. (D) Genetic context of the M. bovis ribosomal operon, indicating known GAM and TYL target genes (23S rRNA, rplD, and rplV).