TABLE 1.
Distribution of antimicrobial susceptibility of 95 Nanopore-sequenced M. bovis isolates and M. bovis PG45 according to ECOFF method (visual estimation, NRI, or ISM)a
| Antimicrobial | Visual estimation |
NRIb |
ISMb (95%/99%) |
||||||
|---|---|---|---|---|---|---|---|---|---|
| ECOFF (μg/ml) | No. |
ECOFF (μg/ml) | No. |
ECOFF (μg/ml)c | No. |
||||
| WT | Non-WT | WT | Non-WT | WT | Non-WT | ||||
| FLOR | >16 | 91 | 4 | >16 | 91 | 4 | >8/16 (+) | 89/91 | 6/4 |
| OTC | >8 | 95 | 0 | >8 | 95 | 0 | >4/8 (+) | 93/95 | 2/0 |
| DOX | >4 | 94 | 0 | >2 | 91 | 3 | >1/2 (+) | 82/91 | 12/3 |
| TIL | ND | >1,024 | ND | ||||||
| TYL | >32 | 46 | 50 | >128d | 46 | 50 | ND | ||
| GAM | >64 | 53 | 43 | >128d | 56 | 40 | ND | ||
| GEN | >16 | 95 | 1 | >8 | 94 | 2 | >4/4 (−) | 91/91 | 5/5 |
| TIA | >0.5 | 90 | 3 | >0.125 | 78 | 15 | >0.06/0.06 (−) | 59/59 | 34/34 |
| ENRO | >2 | 85 | 8 | >1 | 83 | 10 | >1/2 (±) | 83/85 | 10/8 |
a
Determinations of ECOFFs were published previously (13).
b
NRI, normalized resistance interpretation; ISM, iterative statistical method.
c
Plots for residuals were checked and categorized as either good fit (+), poor fit (±), or no fit (−), corresponding to whether the subset values are reliable or not. ND, not possible to determine.
d
Tentative estimate, because the standard deviation was >1.2 log2.