FIG 6.

Propionate or butyrate treatment decreases transcription of the proinflammatory cytokine family CXCL in the setting of AIEC infection of HIE-derived monolayers. Relative transcription of the indicated CXCL family genes measured using the NanoString nCounter inflammation panel. All measurements were performed in DMEM, low glucose alone (CTL) or supplemented with 20 mM propionate (prop) or butyrate (but). The mean of biological triplicates is indicated. Error bars represent the standard deviation. Significance was assessed by a one-way ANOVA with Dunnett’s multiple-comparison test. ****, P ≤ 0.0001; ***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05; ns, not significant.