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. 2021 Sep 24;26(19):5790. doi: 10.3390/molecules26195790

Figure 4.

Figure 4

Vilazodone inhibits L-DOPA effects on spike onset latency after cortical stimulation in striatal MSNs, and this inhibition is mediated by 5-HTr1A stimulation. (A) Schematic illustration of coronal sections through the frontal cortex and middle striatum (approximately at +3.0 and +0.4 mm rostral to bregma) showing the stimulation site in the primary motor cortex (M1), and, collapsed onto one level, the distribution of recording sites in the sensorimotor striatum (shaded) for the different treatment groups (“challenge” units: 6/Veh/LD, red dots; 6/VIL/LD, purple dots; 6/W/VIL/LD, green dots; pooled “baseline” units, black dots). (B) Cortically evoked responses in MSNs of 6-OHDA-lesioned rats recorded before the final drug treatment (“baseline” responses, BL). Displayed are spike onset latency (mean ± SEM) (left) and spike probability (right) in rats that had received a repeated pretreatment with vehicle + L-DOPA (5 mg/kg, i.p.) (6/Veh/LD; n = 11 cells in 5 rats), vilazodone (10 mg/kg, i.p.) + L-DOPA (6/VIL/LD; n = 14 cells in 8 rats), or WAY-100635 (0.5 mg/kg, i.p.) + vilazodone + L-DOPA (6/W/VIL/LD; n = 7 cells in 3 rats). (C) Cortically evoked responses in MSNs of 6-OHDA-lesioned rats recorded after the final drug treatment (“challenge” responses, top). Shown are spike onset latency (left) and spike probability (right) in rats that had received a repeated treatment with vehicle + L-DOPA (5 mg/kg, i.p.) (6/Veh/LD; n = 21 cells in 8 rats), vilazodone (10 mg/kg, i.p.) + L-DOPA (6/VIL/LD; n = 19 cells in 9 rats), or WAY-100635 (0.5 mg/kg, i.p.) + vilazodone + L-DOPA (6/W/VIL/LD; n = 16 cells in 7 rats). Additionally displayed are these data expressed in percentage of baseline values (% BL) (bottom). Vilazodone co-administered with L-DOPA (6/VIL/LD) prevented the L-DOPA-induced decrease in onset latency (6/Veh/LD). WAY administration (6/W/VIL/LD) blocked vilazodone’s ability to attenuate the L-DOPA effect on the onset latency. * p < 0.05, ** p < 0.01, *** p < 0.001, vs. 6/Veh/LD; + p < 0.05, ++ p < 0.01, +++ p < 0.001, vs. 6/VIL/LD.