FIG 5.

Fecal transplantation from db/db mice treated with SB attenuates stroke injury and gut barrier destruction. (a) mNSS was assessed before sacrifice. (b) Representative TTC staining of brain slices and percentages of the cerebral infarct volume in the four groups. (c) Representative images of Nissl staining in the hippocampal CA1 region (scale bar = 100 μm). (d) Double immunostaining for NeuN (neuronal marker) and Iba-1 (microglial marker) was performed in the hippocampal CA1 region, and apoptotic neurons in the peri-infarct cortex were detected using TUNEL staining. DAPI, 4′,6-diamidino-2-phenylindole. (e) The morphology of the ileum and colon was assessed using H&E staining (scale bar = 100 μm). (f) Expression of intestinal tight junction proteins in the four groups. n = 14 to 16 animals per group. Data are presented as mean values ± SD. Asterisks (*) show comparisons with the rWT-Con group, and pound signs (#) show comparisons with the rDb-Con group. *, P < 0.05; **, P < 0.01; ***, P < 0.001; #, P < 0.05; ##, P < 0.01; ###, P < 0.001.