Table 1.

Primer sequences and expected amplicon sizes for gene amplification.

cDNA	Genbank Acc. No.	Forward Primer	Reverse Primer	Amplicon Size (bp)
TNF-α	NM013693.3	5′-ACCTGGCCTCTCTACCTTGT-3′	5′-CCCGTAGGGCGATTACAGTC-3′	161
IL-1β	NM008361.4	5′-GCCACCTTTTGACAGTGATGAG-3′	5′-AGTGATACTGCCTGCCTGAAG-3′	165
IL-6	NM031168.2	5′-CAACGATGATGCACTTGCAGA-3′	5′-TCTCTCTGAAGGACTCTGGCT-3′	201
COX-2	NM011198.4	5′-CCACTTCAAGGGAGTCTGGA-3′	5′-AGTCATCTGCTACGGGAGGA-3′	197
CyclinD2	NM009829.3	5′-ACCTCCCGCAGTGTTCCTATT-3′	5′-CACAGACCTCTAGCATCCAGG-3′	93
CyclinE2	NM001037134.2	5′-TCTGTGCATTCTAGCATCGACTC-3′	5′-AAGGCACCATCGTCTACACATTC-3′	149
p27	NM009875.4	5′-GCGGTGCCTTTAATTGGGTCT-3′	5′-GGCTTCTTGGGCGTCTGCT-3′	230
p53	NM011640.3	5′-ACCGCCGACCTATCCTTACC-3′	5′-TCTTCTGTACGGCGGTCTCTC-3′	118
GAPDH	NM001289726.1	5′-TGTGTCCGTCGTGGATCTGA-3′	5′-TTGCTGTTGAAGTCGCAGGAG-3′	150