Figure 2.

FLT3 protein expression of KG-1a cells after treatment with Dox and combined treatment of Dox–Cur at concentration value of IC₂₀ for 48 h. Protein bands (A) and percentage (C) of FLT3 protein expression level of KG-1a cells treated with DMSO (VC), Dox at concentration of 60 ng/mL, Dox–Cur condition 1 (15 ng/mL Dox + 4.5 µg/mL Cur), Dox–Cur condition 2 (16 ng/mL Dox + 5.5 µg/mL Cur), Dox–Cur condition 3 (12 ng/mL Dox + 7 µg/mL Cur), and Dox–Cur condition 4 (8 ng/mL Dox + 9 µg/mL Cur) for 48 h. Whole protein lysates (80 μg/lane) were loaded onto SDS-PAGE. Protein bands (B) and percentage (D) of FLT3 protein expression level of KG-1a cells treated with DMSO (VC), Cur (4.5 µg/mL), Cur (5.5 µg/mL), Cur (7 µg/mL), and Cur (9 µg/mL) for 48 h. The data are shown as mean ± SD from 3 independent experiments. The significance of mean differences was assessed using one-way ANOVA. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with VC. ^# p < 0.05, ^## p < 0.01, and ^### p < 0.001 compared with single-Dox treatment.