Figure 3.

FLT3 protein levels of KG-1 cells after treatment with Dox and Dox–Cur at concentration value of IC₂₀ for 48 h. Protein bands (A) and percentage (C) of FLT3 protein levels of KG-1 cells were from DMSO (VC), Dox (58 ng/mL), Dox–Cur condition 1 (22 ng/mL Dox + 3.5 µg/mL Cur), condition 2 (10 ng/mL Dox + 4.5 µg/mL Cur), condition 3 (7 ng/mL Dox + 6 µg/mL Cur), and condition 4 (6 ng/mL Dox + 7.5 µg/mL Cur). Protein bands (B) and percentage (D) of FLT3 protein level of KG-1 cells were from DMSO (VC), Cur (3.5 µg/mL), Cur (4.5 µg/mL), Cur (6 µg/mL), and Cur (7.5 µg/mL) for 48 h. Whole protein lysates (80 μg/lane) were loaded onto SDS-PAGE. The data are shown as mean ± SD from 3 independent experiments. The significance of mean differences was assessed using one-way ANOVA. *** p < 0.001 compared with VC. ^### p < 0.001 compared with single-Dox treatment.