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. 2021 Sep 26;26(19):5823. doi: 10.3390/molecules26195823

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Functioning scheme of the AuNPs used in a bio-barcode. (a) Complex formed by a magnetic nanoparticle carrying specific antibodies and a target molecule, attracted on a magnetic substrate. (b) AuNPs bearing a double-stranded DNA and an antibody. (c) The double-stranded DNA washed away by water moves and interacts with a polynucleotide fixed to a chip. (d) Added AuNPs are functionalized by a complementary polynucleotide. (e) Finally, silver deposition allows the amplification of the detection signal.