FIG 4.
EV cytotoxicity is temperature dependent. Human (A) or rabbit (B) erythrocyte hemolysis was assessed by incubating 20 μg of EVs produced at each temperature for either 15 min (for human blood) or 2.5 min (for rabbit blood) at 37°C. The hemolytic activity was determined by reading the absorbance of the samples at OD543. (C) THP-1 cells were incubated with 5 μg of EVs for 4 h prior to cell viability assessment. Data represent means ± SD from 3 independent experiments. ****, P < 0.0001 as calculated by analysis of variance (ANOVA) according to Materials and Methods.