FIG 4.
RS_RS10340 is required for survival in tomato plants. (A) Transposon insertion distribution within RS_RS10340 of transposon insertion libraries in vivo and in vitro. (B) Growth of the ΔRS_RS10340 mutant and wild-type strain GMI1000 in BG medium. (C) Survival curve of tomato plants inoculated with the ΔRS_RS10340 mutant and wild-type strain GMI1000. Kaplan-Meier survival analysis with the Gehan-Breslow-Wilcoxon method was used to compare pathogenicity between the mutant and wild-type strains. A P value of <0.05 was considered significant. (D) The bacterial wilt symptom of tomato plants 9 days postinoculation of the ΔRS_RS10340 mutant and wild-type strain GMI1000. (E) Colonization of the ΔRS_RS10340 mutant. The CFU of R. solanacearum strains in 1 g tomato plant stem were counted 5 days postinoculation. Asterisks indicate significant differences (***, P < 0.001; t test). (F) In vivo competitive index of the ΔRS_RS10340 mutant. The ΔRS_RS10340 mutant and GMI1000Kanr were coinoculated into the stems of tomato plants. The competitive index was measured 5 days postinoculation. Asterisks indicate significant differences (****, P < 0.0001; t test).