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. 2021 Oct 12;12(5):e02180-21. doi: 10.1128/mBio.02180-21

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Copyright © 2021 Böck et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Polar tethering of L. pneumophila correlates with Icm/Dot T4SS structure and function. (A) Representative fluorescence micrographs of D. discoideum Ax3 producing P4C-mCherry (pWS032) and AmtA-GFP (pHK121) and infected (MOI of 5; 2 h) with mCerulean-producing L. pneumophila wild-type or ΔicmT, ΔicmE, ΔicmN, or ΔicmK mutant bacteria harboring plasmid pNP99. Examples are shown for contact between bacteria and the LCV membrane (top, white arrowheads) or no contact (bottom, red arrowheads). Scale bars, 2 μm (overview) and 1 μm (insert). (B) Quantification of data shown in panel A (n^events = 60). Data are represented as mean ± SD from three biological replicates (one-way ANOVA test; *, P < 0.05; **, P < 0.01; ***, P < 0.001).