TABLE 1.
Strains and plasmids used in this study
| Strain/plasmid | Relevant propertiesa | Reference or source |
|---|---|---|
| Strain | ||
| A. castellanii | ||
| 5a2 | ATCC PRA-228 | |
| D. discoideum | ||
| Ax3 | 75 | |
| E. coli | ||
| TOP10 | Invitrogen | |
| L. pneumophila | ||
| GS3007 (ΔicmN) | L. pneumophila JR32 icmN3007::Kanr (lphA) | 10 |
| GS3010 (ΔicmK) | L. pneumophila JR32 icmK3010::Kanr | 10 |
| GS3011 (ΔicmT) | L. pneumophila JR32 icmT3011::Kanr | 45 |
| JR32 | Derivative of wild-type L. pneumophila strain Philadelphia-1 (serogroup 1) | 76 |
| LELA4432 (ΔicmE) | L. pneumophila JR32 icmE::Tn903dIIlacZ | 76 |
| S. Typhimurium | ||
| SL13144 | Virulent SB300 wild-type strain | 77 |
| C. jejuni | ||
| 108 | Virulent patient isolate | 78 |
| Plasmid | ||
| pDM323 | Dictyostelium extrachromosomal expression vector, C-terminal GFP, G418r | 79 |
| pDM1044 | Dictyostelium extrachromosomal expression vector, C-terminal mCherry, Hygr | 80 |
| peGFP-2×FYVE | Mammalian expression vector, fluorescent PtdIns(3)P probe | 81 |
| pHK121 | pDM323-amtA-gfp | 48 |
| pNP99 | L. pneumophila extrachromosomal expression vector, mCerulean, Camr | 47 |
| pWS032 | pDM1044-P4CSidC-mCherry | 47 |
a
Cam, chloramphenicol; Hyg, hygromycin; Kan, kanamycin; G418, Geneticin.