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. Author manuscript; available in PMC: 2021 Oct 12.
Published in final edited form as: Addict Biol. 2020 Mar 16;26(2):e12889. doi: 10.1111/adb.12889

FIGURE 7.

FIGURE 7

PLX5622 diet depletes other peripheral macrophage and monocyte populations. C57BL/6J male mice were on either control diet or PLX5622 diet for 21 days to deplete microglia. Spleen, blood, and liver were collected from both groups. A, Representative flow cytometry plots of total CD45+, live, and myeloid (CD11c+ and/or CD11b+) cells that are macrophages (CD64+F4/80+) (top) or monocytes (CSF1R+CD11b+) from the spleens of control diet– and PLX5622 diet–treated mice. B, Representative flow cytometry plots of total CD45+, live, and myeloid (CD11c+ and/or CD11b+) cells that are macrophages (CD64+F4/80+) (top) or monocytes (CSF1R+CD11b+) from the blood of control diet– and PLX5622 diet–treated mice. C, Representative flow cytometry plots of total CD45+, live, and myeloid (CD11c+ and/or CD11b+) cells that are macrophages (CD64+F4/80+) (top) or monocytes (CSF1R+CD11b+Gr1lo) from the livers of control diet– and PLX5622 diet–treated mice. D-I, Graphs show mean ± SEM of accumulative data (n = 3/group). Graphs show the frequency of myeloid cells that are CD64+F4/80+ macrophages from the (D) spleens, (E) blood, and (F) livers of the indicated treatment groups. Graphs show the frequency of myeloid cells that are CSF1R+CD11b+Gr1lo monocytes from the (G) spleens, (H) blood, and (I) livers of the indicated treatment groups. Data were analyzed by Welch t test (*P < 0.05, **P < 0.01, ***P < 0.001).