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. 2021 Oct 12;12(5):e02530-21. doi: 10.1128/mBio.02530-21

FIG 4.

FIG 4

Polymeric O antigen shields CDI receptors. (A) LPS was isolated from the indicated E. coli MG1655 strains, resolved by SDS-PAGE, and stained with Pro-Q Emerald LPS stain. The LPS standard (from E. coli serotype O55:B5) was provided in the stain kit. (B) E. coli MG1655 cells expressing either CdiASTEC3 or CdiASTEC4 were cocultured at a 1:1 ratio with E. coli MG1655 target bacteria in broth. Where indicated, inhibitor and/or target strains carried plasmid pMF19 to restore O16 antigen (O-ag) synthesis. (C) The inhibitor and target cell strains from panel B were cocultured at a 1:1 ratio on agar media. Competitive indices are the averages ± SEM from three independent experiments.