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. 2021 Sep 28;13:703984. doi: 10.3389/fnagi.2021.703984

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Copyright © 2021 Nakagaki, Nishida and Satoh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Real-time quaking-induced conversion of α-synuclein. (A) Outline of RT-QuIC. Tissues or body fluids of patients are added to a reaction buffer that contains recombinant α-synuclein (rec α-syn). Aggregated α-syn converts rec α-syn to the abnormal structure, which forms amyloid by repeated shaking and incubation. Thioflavin T (ThT) binds to the β-sheet structure of amyloid and its fluorescence is detected. (B) Results of α-syn RT-QuIC. The left panel shows the result of a brain homogenate from an α-synucleinopathy patient and the right panel shows the result of a non-α-synucleinopathy patient.