Figure 1.

The F. nucleatum was enriched in colorectal cancer and enhanced CRC cells invasion and migration. (a) The fecal abundance of F. nucleatum among the healthy and CRC patients in the GMrepo database (Mann-Whitney test). (b) Fecal bacteria genomic DNA was extracted from the healthy population (n = 59), CRC patients (n = 63) in cohort 1. The abundance of F. nucleatum was examined by qPCR. (Cohort 1, Mann-Whitney test). (c) Bacteria genomic DNA was extracted from CRC tissues and adjacent normal mucosa (n = 77) in cohort 2. The abundance of F. nucleatum was tested by qPCR. (d) The abundance of F. nucleatum among CRC patients with different TNM stage in our tested CRC tissues (One-way ANOVA test). (e, f) After incubated with PBS or E. coli or F. nucleatum, the mobility of HCT116 and LoVo cells was detected by wound healing assay (e) or transwell assay (f). Every 6 fields were counted for each sample (Each experiment was repeated in triplicate; Student’s t test). Scale bars:100 μm. * p < .05, ** p < .01, and *** p < .001, **** p < .0001, ns no significant