Figure 3. Memory extinction (A) Testing and training protocols for B,C.

Training + Extinction: larvae were exposed to 18 cycles of alternating CO₂ and air following training. Habituation + Training: larvae were exposed to 18 cycles of alternating CO₂ and air prior to training. (B) Probability of choosing CO₂ containing channel (top) and fraction of larvae in trained group according to double Gaussian model fit (bottom) before and after training scheme. (C) Histograms of individual larva preference after training, for all larva and for larva trained with 2–4 training cycles. * p<0.05, ** p<0.01, *** p<0.001.

Figure 3—figure supplement 1. After extinction, larvae can be trained again.

(A) Testing and training protocol for B,C. Larvae were trained with three cycles of paired training, followed by 18 extinction cycles of alternating CO₂ and air with no reward presented. After extinction, larvae were presented with three additional paired training cycles before testing. (B) Probability of choosing CO₂ containing channel before and after training scheme. (C) Fraction of larvae in trained group according to double Gaussian model fit before and after training scheme. All larvae were DAN-i1>CsChrimson and raised on ATR+ food. *** p<0.001.

Figure 3—figure supplement 2. Larvae population average response following training.

(A) Larvae population average response in the first ten minutes following training (0–10 min), compared to the latter fifty minutes of testing (10–60 min), for larvae that had been given 2, 5, or 20 cycles of paired training. (B) Larvae population average response for the first five choices made by the larvae following training, compared to the remaining choices, for larvae that had been given 2, 5, or 20 cycles of paired training and made at least 10 decisions following training. (C,D,E) Larvae population average response over 15-min segments following training, for larvae trained with (C) 2 cycles, (D) 5 cycles, or (E) 20 cycles of training. All larvae were DAN-i1>CsChrimson and raised on ATR+ food.

Figure 3—figure supplement 3. Larvae given additional training between testing periods.

(A) Testing and training protocols for experiments in B. All larvae are tested in the Y-maze for one hour to determine initial preference. Larvae were then trained with 10 cycles of paired training, followed by a 15-min test period. The 10 cycle train/15 min test was repeated four times. (B) Probability of choosing CO₂-containing channel before training, and during each of the four test periods. All larvae were DAN-i1>CsChrimson and raised on ATR+ food. *** p <0.001.