Table 3. p-Values for experiments in Figure 1, Figure 2, Figure 3, and Figure 4.
P-values for experiments were calculated: Bootstrap - p-values calculated as explained in Materials and methods; Fisher - p-values calculated using Fisher’s exact test; U-test - p-values calculated using two-sided Mann–Whitney U test. Unless otherwise noted, p-values are calculated between pre-train and post-train data. A shaded row indicates not all tests reach the same significance level (out of ns, p <0.05, p <0.01, p <0.001).
| Experiment | Genotype | Hierarchical Bootstrap | Bootstrap Animal Only | Fisher | U-test |
|---|---|---|---|---|---|
| Figure 1B | |||||
| Gr63a1/DANi1> CsChrimson, ATR+ | <10−4 | <10−4 | <10−4 | <10−4 | |
| Gr63a1/DANi1> CsChrimson, ATR- | <10−4 | <10−4 | <10−4 | <10−4 | |
| Figure 1D | |||||
| Paired | DANi1> CsChrimson | <10−4 | <10−4 | <10−4 | <10−4 |
| Offset After | DANi1> CsChrimson | <10−4 | <10−4 | <10−4 | <10−4 |
| Reverse Paired | DANi1> CsChrimson | 0.3429 | 0.2689 | 0.6166 | 0.9379 |
| Offset Before | DANi1> CsChrimson | 0.4479 | 0.4373 | 0.9479 | 0.9770 |
| Paired, ATR- | DANi1> CsChrimson | 0.4762 | 0.4315 | 1.000 | 0.2658 |
| No Training | DANi1> CsChrimson | 0.4066 | 0.3664 | 0.7726 | 0.9835 |
| DAN w/o CO2 | DANi1> CsChrimson | 0.3935 | 0.3102 | 0.7173 | 0.4852 |
| Driver ctrl | SS00864 | 0.3106 | 0.0313 | 0.3411 | 0.3977 |
| Effector ctrl | UAS-CsChrimson | 0.3383 | 0.2361 | 0.6336 | 0.8366 |
| 58E02> CsChrimson | 58E02> CsChrimson | <10−4 | <10−4 | <10−4 | <10−4 |
| Figure 1C | DANi1> CsChrimson | ||||
| Forward Paired | <10−4 | <10−4 | <10−4 | <10−4 | |
| Backwards Paired | 0,3368 | 0.163 | 0.6801 | 0.1939 | |
| Btw CO2 | 0.0107 | 0.0001 | 0.006543 | 0.0003257 | |
| Figure 1D | DANi1> CsChrimson | ||||
| 6.5% | <10−4 | <10−4 | <10−4 | <10−4 | |
| 8% | <10−4 | <10−4 | <10−4 | <10−4 | |
| 15% | <10−4 | <10−4 | <10−4 | <10−4 | |
| 18% | <10−4 | <10−4 | <10−4 | <10−4 | |
| Figure 2A | DANi1> CsChrimson | ||||
| 0 Cycles | 0.4132 | 0.3647 | 0.7726 | 0.9835 | |
| 1 Cycles | 0.0003 | <10−4 | <10−4 | 0.0591 | |
| 2 Cycles | <10−4 | <10−4 | <10−4 | <10−4 | |
| 3 Cycles | <10−4 | <10−4 | <10−4 | <10−4 | |
| 4 Cycles | <10−4 | <10−4 | <10−4 | <10−4 | |
| 5 Cycles | <10−4 | <10−4 | <10−4 | <10−4 | |
| 10 Cycles | <10−4 | <10−4 | <10−4 | <10−4 | |
| 20 Cycles | <10−4 | <10−4 | <10−4 | <10−4 | |
| Figure 3B | DANi1> CsChrimson | ||||
| 2 Cycles, Training | <10−4 | <10−4 | <10−4 | <10−4 | |
| 2 Cycles, Habituation + Training | <10−4 | <10−4 | <10−4 | <10−4 | |
| 2 Cycles, Training + Extinction | 0.0117 | 0.0020 | 0.001339 | 0.04743 | |
| 3 Cycles, Training | <10−4 | <10−4 | <10−4 | <10−4 | |
| 3 Cycles, Habituation + Training | <10−4 | <10−4 | 0.0007459 | <10−4 | |
| 3 Cycles, Training + Extinction | 0.1133 | 0.0176 | 0.1763 | 0.03069 | |
| 4 Cycles, Training | <10−4 | <10−4 | <10−4 | <10−4 | |
| 4 Cycles, Habituation + Training | <10−4 | <10−4 | <10−4 | <10−4 | |
| 4 Cycles, Training + Extinction | <10−4 | <10−4 | <10−4 | <10−4 | |
| 5 Cycles, Training | <10−4 | <10−4 | <10−4 | <10−4 | |
| 6 Cycles, Habituation + Training | <10−4 | <10−4 | <10−4 | <10−4 | |
| 6 Cycles, Training + Extinction | <10−4 | <10−4 | <10−4 | <10−4 | |
| 10 Cycles, Training | <10−4 | <10−4 | <10−4 | <10−4 | |
| 10 Cycles, Habituation + Training | <10−4 | <10−4 | <10−4 | <10−4 | |
| 10 Cycles, Training + Extinction | <10−4 | <10−4 | <10−4 | <10−4 | |
| Figure 4B | DANi1> CsChrimson | ||||
| 20x Pre-Test/Post-Test | <10−4 | <10−4 | <10−4 | <10−4 | |
| 20x Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| 20x (Only Test Next Day) Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| 5x Pre-Test/Post-Test | <10−4 | <10−4 | <10−4 | <10−4 | |
| 5x Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| 2x Pre-Test/Post-Test | <10−4 | <10−4 | <10−4 | <10−4 | |
| 2x Pre-Test/Next Day | 0.2086 | 0.0501 | 0.3524 | 0.07216 | |
| 2x (Only Test Next Day) Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| No Train Pre-Test/Post-Test | 0.4035 | 0.3319 | 0.7893 | 0.2003 | |
| No Train Pre-Test/Next Day | 0.1583 | 0.0530 | 0.3071 | 0.8884 | |
| RP 20x Pre-Test/Post-Test | 0.2677 | 0.1507 | 0.4276 | 0.7396 | |
| RP 20x Pre-Test/Next Day | 0.4205 | 0.3481 | 0.8474 | 0.3765 | |
| Ext Post-Train Pre-Test/Next Day | 0.1801 | 0.0146 | 0.3315 | 0.01336 | |
| Ext Pre-Test Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| Figure 4C | DANi1> CsChrimson | ||||
| M 20x (CXM+/ATR+) Pre-Test/Post-Test | <10−4 | <10−4 | <10−4 | <10−4 | |
| M 20x (CXM+/ATR+) Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| M 20x (CXM-/ATR+) Pre-Test/Post-Test | <10−4 | <10−4 | <10−4 | <10−4 | |
| M 20x (CXM-/ATR+) Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| S 10x (CXM+/ATR+) Pre-Test/Next Day | 0.1099 | 0.014 | 0.1671 | 0.02985 | |
| S 10x (CXM-/ATR+) Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| M 10x (CXM+/ATR+) Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| M 10x (CXM-/ATR+) Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| Figure 4D | DANi1> hs-dCREB2-b;CsChrimson | ||||
| M 10x, HS Pre-Test/Post-Test | <10−4 | <10−4 | <10−4 | <10−4 | |
| M 10x, HS Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| M 10x, No HS Pre-Test/Post-Test | <10−4 | <10−4 | <10−4 | <10−4 | |
| M 10x, No HS Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 | |
| S 10x, HS Pre-Test/Post-Test | 0.3804 | 0.2830 | 0.7310 | 0.2750 | |
| S 10x, HS Pre-Test/Next Day | 0.2645 | 0.08860 | 0.4650 | 0.3802 | |
| S 10x, No HS Pre-Test/Post-Test | <10−4 | <10−4 | <10−4 | <10−4 | |
| S 10x, No HS Pre-Test/Next Day | <10−4 | <10−4 | <10−4 | <10−4 |