| Other |
Wildtype C57BL/6 J (Mus musculus) |
The Jackson Laboratory |
Stock number: 000664 |
Experimental Animal Facility, Institute of Biomedical Sciences, Academia Sinica IACUC 12-03-332, 20-06-1492 |
| Other |
Asic1a-/-(specific targeting alternative spliced isoform Asic1a-/-) C57BL6/J (Mus musculus) |
Eur J Neurosci 2015 Jun; 41(12):1553–68. doi:10.1111/ejn.12905. |
Asic1
-/-
|
Experimental Animal Facility, Institute of Biomedical Sciences, Academia Sinica IACUC 12-03-332, 20-06-1492 |
| Other |
Asic3-/- C57BL6/J (Mus musculus) |
Nat Commun 2016 May; 7:11,460. doi:10.1038/ncomms11460. |
Asic3
-/-
|
Experimental Animal Facility, Institute of Biomedical Sciences, Academia Sinica IACUC 12-03-332, 20-06-1492 |
| cell line (Cricetulus griseus) |
Epithelial like Chinese Hamster ovary cells |
ATCC |
CCL-61 (RRID:CVCL_0214) |
|
| Transfected construct (Discosoma sp.) |
pCMV - mCherry |
Gift from Dr. Huang, Yi-Shuian, IBMS, Academia Sinica, Taiwan |
Vector control |
1 μg DNA: 3 μl Lipofectamin 2000 in 1 ml opti-MEM medium |
| Transfected construct (Mus musculus) |
pT7-RFP-N2- T▲T - P2A - mASIC1a (alternative spliced isoform Asic1a) |
The cDNA of mouse ASIC1a was subcloned into the plasmid (pT7-RFP-N2) |
Asic1
|
1 μg DNA: 3 μl Lipofectamin 2000 in 1 ml opti-MEM medium |
| Antibody |
pERK; Rabbit polyclonal [polyclonal Phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204)] |
Cell Signaling Technology |
#9,101 (RRID:AB_331646) |
IHC (1:500) |
| Antibody |
Doublecortin; (Rabbit polyclonal) |
Cell Signaling |
#4,604 (RRID:AB_561007) |
ICC (1:200) |
| Antibody |
MAP2; (Mouse monoclonal) |
Thermo Fisher Scientific |
MA5-12823 (RRID:AB_10982160) |
ICC (1:200) |
| Antibody |
Secondary biotinylated goat-anti-rabbit antibodies; (Goat monoclonal) |
Vector Laboratories |
BA-1000–1.5 (RRID:AB_2313606) |
(1:1000) |
| Antibody |
Alexa Fluor 488-conjugated goat anti-rabbit IgG; (Goat monoclonal) |
Thermo Fisher Scientific |
16–237 (RRID:AB_436053) |
(1:100) |
| Antibody |
Alexa Fluor 555-conjugated goat anti-mouse IgG; (Goat monoclonal) |
Thermo Fisher Scientific |
A-21422 (RRID:AB_141822) |
(1:100) |
| Commercial assay or kit |
IHC Reagent; Avidin-Biotin pre-mix solution |
Vector Laboratories |
A-2004–5 (RRID:AB_2336507) |
(1:200) |
| Commercial assay or kit |
IHC staining kit; DAB Peroxidase Substrate Kit with Nickel, 3,3’-diaminobenzidine |
Vector Laboratories |
SK-4100 (RRID:AB_2336382) |
According to instruction manual |
| Commercial assay or kit |
Live cell imaging staining reagent for calcium; Invitrogen Oregon Green 488 BAPTA-1, AM, cell permeant |
Thermo Fisher Scientific |
O6807 |
5 μM |
| Commercial assay or kit |
Live cell imaging staining reagent for calcium; Invitrogen Fluo-4, AM, FluoroPure |
Thermo Fisher Scientific |
F23917 |
5 μM |
| Commercial assay or kit |
Live cell imaging staining reagent for calcium; Fura-2, AM, cell permeant |
Thermo Fisher Scientific |
F1221 |
5 μM |
| Chemical compound, drug |
GsMTx-4 |
Abcam Inc |
ab141871 |
500 nM |
| Chemical compound, drug |
Amiloride |
Sigma-Aldrich |
A7410-1G |
100 μM |
| Chemical compound, drug |
PcTx1 |
Tocris |
#5,042 |
0.1–50 nM |
| Chemical compound, drug |
Ruthenium Red |
Tocris |
#1,439 |
5–10 μM |
| Chemical compound, drug |
RyR inhibitor, JTV519 fumarate |
Tocris |
#4,564 |
10 μM |
| Chemical compound, drug |
Thapsigargin |
Sigma-Aldrich |
T9033 |
100 nM |
| Chemical compound, drug |
Cytochalasin D |
Tocris |
#1,233 |
5 μg- 10 μg/ml |
| Chemical compound, drug |
Nocodazole |
Tocris |
#1,228 |
5 μg- 10 μg/ml |
| Software, algorithm |
Image J |
Abràmoff, M. D., Magalhães, P. J., & Ram, S. J. (2004). Image processing with ImageJ. Biophotonics international, 11(7), 36–42. |
Particle analysis |
Set threshold and define particle sizes |
| Software, algorithm |
GraphPad Prism 8 |
Swift, M. L. (1997). GraphPad prism, data analysis, and scientific graphing. Journal of chemical information and computer sciences, 37(2), 411–412. |
Student t-test |
|
