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MSH-capture ELISA: typical standard curves for MS-MPB in 12.5% acetonitrile in TBS (used for samples biotinylated by the acetonitrile extraction procedure) or in 22 mM phosphate buffer (pH 8.0) without acetonitrile (used for samples biotinylated by the chloroform extraction procedure). Data shown are from 40 min of development. Error bars indicate the standard deviation from the mean (n = 3).
