Table I.
Primer sequences used for reverse transcription-quantitative PCR.
| Gene | Species | Primer sequence, 5′→3′ |
|---|---|---|
| SETD7 | Mouse | Forward TCCAAGGTAGCAGTTGGACCTAA |
| Reverse CCAAGGAGGCACAGTACTTGG | ||
| YAP | Mouse | Forward ATTTCGGCAGGCAATACGGA |
| Reverse TGCTCCAGTGTAGGCAACTG | ||
| HIF-1α | Mouse | Forward AGCACAGTTACAGTATTCCAGCAGAC |
| Reverse TCATCAGTGGTGGCAGTGGTAGT | ||
| COL2A1 | Mouse | Forward GGCCAGGATGCCCGAAAATTA |
| Reverse GGCTGCAAAGTTTCCTCCAC | ||
| SOX9 | Mouse | Forward CTCCTACTACAGCCACGCAG |
| Reverse GCTGTGTGTAGACGGGTTGT | ||
| Aggrecan | Mouse | Forward AGCTTGAGGGGGAAGTGTTC |
| Reverse GGTTGACGATGGGGTATCGG | ||
| GLUT1 | Mouse | Forward ACCACCTCACTCCTGTTACTTACCT |
| Reverse ATCCAAACCTCCTACCCTCAATCCA | ||
| LDHA | Mouse | Forward TAGGCTACAACAGGATTCTAGGTGGAG |
| Reverse GTCAGAGGTGGCAGAACTATTTC | ||
| PGK1 | Mouse | Forward GAGCCAAGTCGGTAGTCCTTATGAG |
| Reverse CACAGTCCTTCAAGAACAGAACATCCT | ||
| PKM2 | Mouse | Forward GTGCCGCCTGGACATTGATTCA |
| Reverse AGTTCAGACGAGCCACATTCATTCC | ||
| 18S | Mouse | Forward GGCGCCCCCTCGATGCTCTTAG |
| Reverse GCTCGGGCCTGCTTTGAACACTCT | ||
| GAPDH | Mouse | Forward TTGCAGTGGCAAAGTGGAGA |
| Reverse GATGGGCTTCCCGTTGATGA |
SETD7, SET domain containing 7; YAP, Yes-associated protein; HIF-1α, hypoxia inducible factor-1α; COL2A1, collagen II, α1; SOX9, SRY-related box gene 9; GLUT1, glucose transporter 1; LDHA, lactate dehydrogenase A; PGK1, phosphoglycerate kinase 1; PKM2, pyruvate kinase isoenzyme 2.