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. 2021 Oct 13;11(10):210132. doi: 10.1098/rsob.210132

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© 2021 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 2. — Isotropicity and the expansion factor. (a) Non-expanded and expanded PCF cells stained with α-tubulin (magenta; Alexa Fluor 647), kDNA and nuclear DNA (cyan; DAPI). Imagery was deconvolved using Huygens Professional and visualized using Imaris. (b,c) Magnified views and measurements of the cytoskeleton length, kDNA length and nucleus diameter in non-expanded and expanded cells were done in ImageJ. Expansion factor was calculated as the ratio between non-expanded and expanded cell compartments.