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. 2021 Oct 13;11(10):210132. doi: 10.1098/rsob.210132

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© 2021 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — Representative confocal images of kDNA during different stages of the cell cycle. (a) Comparative confocal images of expanded and non-expanded T. brucei kDNA (DAPI, cyan). (b) Representative images of the kDNA and basal bodies during different kDNA replication steps. DNA stained with DAPI (cyan); cytoskeleton stained with a monoclonal alpha-tubulin antibody (magenta; Alexa Fluor 647). Asterisk marks the ‘cloudy’ part of the kDNA. Imagery was deconvolved using Huygens Professional and visualized using Imaris.