TABLE 4.
Results of PCR-mediated identification
| Organisma | No. of strains | No. (%) identified with the following primer pair:
|
PCR-RFLP resultsd
|
||
|---|---|---|---|---|---|
| PC1-PC2b | PC480-PC1250c | Type | No. (%) of strains with the indicated type | ||
| B. cepacia | 50 | 22 (44) | 50 (100) | AAAB | 20 (40) |
| AAAA | 19 (38) | ||||
| ABBB | 11 (22) | ||||
| B. gladioli | 14 | 0 | 14 (100) | BBBC | 10 (71) |
| BBEC | 4 (29) | ||||
| R. pickettii | 6 | 0 | 0 | DDDE | 2 (100) |
| B. multivorans | 2 | 0 | 2 (100) | AAAA | 2 (100) |
| S. maltophilia | 3 | 0 | 0 | CCCD | 3 (100) |
| P. aeruginosa | 11 | 0 | 0 | EEFH | 3 (27) |
| FEFH | 6 (55) | ||||
| GFFH | 2 (18) | ||||
| Clinical isolatee | 27 | 2 (7) | 21 (78) | AAAA | 18 (67) |
| AAAB | 2 (7) | ||||
| EEFH | 5 (19) | ||||
| Other | 2 (7) | ||||
a
Identification as provided by the strain contributors.
b
Increased level of discordance for B. cepacia compared to results with PC1250-PC480, but correctly negative for the other species.
c
Apparently suited for identifying both Burkholderia species; however, non-Burkholderia species also may be identified.
d
Order of restriction enzymes used: AluI, CfoI, MspI, and DdeI.
e
RFLP was considered the gold standard for identification.