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. 2021 Oct 5;2021:9955717. doi: 10.1155/2021/9955717

Figure 2.

Figure 2

The effect of MICAL2 on the proliferation of gastric cancer cells. (a, b) BGC-823 (a) and SGC-7901 cells (b) were transfected with control siRNA or siRNA specifically targeting MICAL2 (siMICAL2). After 48 h, total protein extracts from cells were analyzed for MICAL2 protein expression. Western blot bands corresponding to MICAL2 were quantified and normalized against GAPDH levels. (c) SGC-7901 cells were transfected with empty vector or MICAL2 overexpression plasmids, and total cellular proteins were extracted and analyzed for MICAL2 expression by Western blotting. Data in (a–c) are presented as mean ± SEM of 3 determinations. ∗P < 0.05, ∗∗∗P < 0.001. (d–f) The viability of BGC-823 and SGC-7901 cells transfected with siMICAL2 (d, e) and SGC-7901 cells transfected with MICAL2 overexpression plasmids (f) was assessed by cell counting kit-8 (CCK-8) assay. Data are presented as mean ± SEM of 5 determinations. (d, e) ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. siCtrl. (f) ∗P < 0.05, ∗∗∗P < 0.001 vs. the empty vector. (g, h) Representative images of EdU staining in BGC-823 cells transfected with siMICAL2 (g) and SGC-7901 cells transfected with MICAL2 overexpression plasmids (h), and the cell proliferation rate was quantified. Data are presented as mean ± SEM of 5 determinations. Scale bar, 5 μm. ∗∗∗P < 0.001.