FIG. 3.

In vivo synthesis of small RNA species after repression of RPC17 gene expression. Strain YMLF2 harboring plasmid pCMc17, which allows the modulation of RPC17 expression by changing doxycycline concentration in the growth medium, was grown at 30°C with (+) or without (−) the addition of doxycycline (5 μg/ml). Tritiated uracil incorporation was allowed for 10 min after 6 or 12 h of growth, as indicated. RNA species were extracted and analyzed by electrophoresis on a 7 M urea gel (6% polyacrylamide), using equal amounts of RNA (6 μg per lane). (A) Ethidium bromide staining of RNA; (B) autoradiography of the dried gel shown in panel A.