Fig. 6. FBXW11 mediates stem-cell-like properties of CRC cells via SIRT1.

HCT116 and SW620 cells were co-transfected with recombinant lentiviral vectors carrying FBXW11 (or empty control vectors) and shRNA targeting SIRT1 (or scrambled shRNA sequence). A Cell viability at different time points after transfection was evaluated by the MTT assay. B, E The wound-healing assay was used to assess the migration capacity of CRC cells following transfection. The wound closure at 24 h after scratching was measured. C, F The invasion capacity of transfected CEC cells was determined by the Transwell assay. The number of invaded cells per field at 24 h post plating was calculated. D, G The sphere-forming ability of CRC cells following transfection was evaluated by the sphere formation assay. The number of spheres with a diameter of over 50 μm was counted. H The protein expressions of Nanog, ALDH1, and Oct4 were assessed by western blotting. ANOVA followed by Bonferroni’s post hoc test was used for statistical comparisons among multiple groups.