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. 2021 Oct 12;12(10):934. doi: 10.1038/s41419-021-04235-0

Fig. 1. LPS stimuli promoted de novo fatty acid synthesis pathway in macrophages.

Fig. 1

A, B Murine bone marrow-derived macrophages (BMDMs) or murine RAW 264.7 macrophages were cultured in the absence or presence of 100 ng/ml lipopolysaccharide (LPS). The protein levels of fatty acid synthase (FASN), acetyl-CoA carboxylase (ACACA), and ATP citrate lyase (ACLY) were determined by Western blotting. Representative images of western blotting (A) and a statistical analysis of relative protein levels (B). C The intracellular free fatty acid (FFA) was determined by free fatty acid assay kit after exposure to LPS (n = 4). Data were all represented as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, t-test.