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. 2021 Oct 12;12(10):934. doi: 10.1038/s41419-021-04235-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Macrophages in the presence of LPS were treated with metformin, C75 or Fasn shRNA lentivirus. A–C The membrane localization of Akt in response to a 15-min LPS treatment was determined by immunofluorescence (A–B) and Western blotting (C). D, E Akt possessed a posttranslational modification called palmitoylation, as determined in RAW 264.7 by acly-biotin exchange (ABE) method. The palmitoylation of exogenously-overexpressed Akt (D) and endogenous Akt (E). F–H The effect of metformin and FASN inhibition on Akt palmitoylation was determined by ABE method (F) and then confirmed again by immunoprecipitation (IP)-ABE method (G). The ratio of palmitoylated Akt/Akt was quantified (H). Data were represented as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ANOVA.