Fig. 1. Development of a pipeline for scRNA/cfDNA from longitudinally sampled CSF samples before and after ICI therapy.

a Schematic representation of the longitudinal sampling performed on patients in this study. b Longitudinal sampling overview from patients in each study, including trial primary endpoint (dashed line), and date of patient mortality, when known. c UMAP of single-cell transcriptomes from all captured CSF cells in both trials, colored by patient, with cell type of origin indicated.