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. 2021 Oct 12;12:5955. doi: 10.1038/s41467-021-25860-5

Fig. 4. Longitudinal scRNA and cfDNA from P043 suggest the adaptive selection of a less-immunogenic over a more-immunogenic subclone.

Fig. 4

a Tumor fraction within CSF, as measured by Seq-Well and cytology, overlaid with tumor purity inferred by ABSOLUTE run on CSF-derived cfDNA. b MRI imagery at 0, 6, and 12 weeks relative to treatment; LMD-indicative enhancement indicated by red arrow. c Unsupervised clustering of inferred copy number profiles (left, see Methods) and expression (right) reveals intercellular heterogeneity, possibly explainable by the presence of subclones. d Relative proportions of subclones as a function of time. Darker purple and lighter purple denote the descendant and ascendant subclone, respectively. e IFN-γ response expression in subclones over time (***p = 0.001, **p = 0.01, Wilcoxon ranked-sum test, Cohen’s d = 1.4, N = 52 for descendent, 19 for ascendant at P043-3; Cohen’s d = 1.4, N = 14 for descendent, 31 for ascendant at P043-4). Darker purple and lighter purple denote the descendant and ascendant subclone, respectively.