Fig. 1. Design and expression screening of Pro-6C chimeras.

a Schematic representation of Pro-6C and different linker sequences. A panel of linker sequences (A1–C4) were inserted between the two domains. b Coomassie blue-stained 4–12% polyacrylamide gel of purified proteins from 0.5 L-scale fermentation. Ref: Pro-6C. c Immune-blotting analysis of the same gel using the conformational reduction-sensitive mAb45.1 as primary antibody. All blots and gel are derived from the same experiment and processed in parallel. Composite images are presented in panels (a) and (b) from original images files provided in Supplementary Figure S4 and S5, respectively.