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. 2021 Oct 12;6:120. doi: 10.1038/s41541-021-00383-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Reversed-phase (RP) HPLC. Reversed-phase HPLC–UV chromatogram recorded following analysis of purified ProC6C. The peak at 14.14 ml corresponds to monomeric ProC6C antigen. b Size-exclusion chromatography (SEC) HPLC analysis. SEC-HPLC analysis was performed under native conditions in a phosphate buffer pH 6.7 to determine the amount of monomer in the sample. c SEC-MALS analysis. ProC6C was injected onto a Superdex 200 Increase 10/300GL column and the change in refractive index as a function of protein concentration was used to compute the molar masses. The solid line plotted on the right axis corresponds to the change in refractive index from the SEC column. The right axis is normalized to the greatest magnitude across the chromatogram data, i.e., to the monomer peak of ProC6C. The molar masses across the eluting peak are plotted as open circles on the left axis (molar mass). The average molecular mass is indicated. d Distributions of hydrodynamic radii obtained from dynamic light scattering (DLS) on ProC6C at three different temperatures.