Fig. 5. Immunogenicity and functional activity of ProC6C vaccine formulations.

Groups of mice were immunized with ProC6C formulated with Alhydrogel^® alone, Matrix-M adjuvant alone, and Alhydrogel® together with Matrix-M adjuvant. a Levels of vaccine-specific antibodies in individual mice are shown as midpoint titers. Midpoint titers below 50 are reported as 50. Bars represent median values. b Pooled sera were tested in SMFA at 1:9 dilution. Reported values and 95% confidence intervals (bars) are determined by general linearized mixed models and used oocyst count data from two independent SMFA experiments with 20 mosquitoes per condition and experiment. Transmission-reducing activity is calculated by comparing with a nonserum control included in each SMFA. c Levels of CSP-specific antibodies in individual mice are shown as midpoint titers. Midpoint titers below 50 are reported as 50. Bars represent median values. d Inhibition of sporozoite invasion of human hepatocytes (HC-04) by 1:20-diluted heat-inactivated mice sera. Dots are means of three independent replicates in each experiment, and bars are means of the two independent experiments. Preimmune mouse serum (Pre) and 100 µg/mL anti-CSP antibody 2A10⁴⁶ were included as negative and positive controls, respectively.