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. 2021 Oct 12;10(10):67. doi: 10.1038/s41389-021-00356-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A The colocalization of TRPC1, CaM, and PI3K in CRC cells using immunofluorescence analysis. The representative immunohistological staining of TRPC1, CaM, and PI3K in the HCT116, HT29, and SW620 cell lines (left panel) and the traces of fluorescence intensity spatial profiles (right panel). B The representative immunohistological staining of TRPC1, CaM, and PI3K in human colorectal tumor tissues and adjacent tissues from the local cohort (left panel) and the traces of fluorescence intensity spatial profiles (right panel). Nuclear were stained with DAPI. C Trpc1 knockout inhibited the colocalization of CaM and PI3K. Left panel, the representative immunohistological staining of TRPC1, CaM, and PI3K in CRC tumor tissues in the AOM/DSS-WT and AOM/DSS-Trpc1^-/- mice. Right panel, the traces of fluorescence intensity spatial profiles. D, E The interaction of TRPC1, CaM, and PI3K p85 subunit was confirmed by co-immunoprecipitation assay in SW620 cells. Typical results are shown in the left panel along with the statistical analysis in the right panel (n = 3). *P < 0.05 vs. shControl group; ^##P < 0.01 vs. vector group.