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. 2021 Oct 12;11:20231. doi: 10.1038/s41598-021-99602-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Stable colonization of E. gallinarum bearing lysogenic phiEG37k phage produces residual phage particles in the colon. C57BL/6 mice that have Altered Shaedler Flora (ASF-mice) were gavaged E. gallinarum either free from (G0 strain) or bearing lysogenic phiEG37k (G2 strain). (a and b) Titers of E. gallinarum (a) and phiEG37k (b) in feces from the ASF mice with no Enterococcus colonization (Ctrl), G0-colonization or G2-colonization at 4 weeks post-gavage. (c) Components of colonic microbiota in mice of the three groups described in (a). (d) Abundancy of individual bacteria consisting of ASF or E. gallinarum in the colon from mice described in (a) was displayed. One way analysis of variance with Tukey’s multiple comparison was performed for ad-hoc analysis. ns; not significant. n = 6–7.