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. 2021 Oct 12;4:1181. doi: 10.1038/s42003-021-02714-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Binding of human B cells to AM by flow cytometry for a subject T1 (CD20+, CD27+, IgG+), b subject L1 (CD19+, IgG+). Binding of mAbs to H37Rv AM by ELISA at varying concentrations from c subject T1, and d subject L1. Florescence-minus-one (FMO) controls were used to set conservative AM+ gates and IgG+ gates for sorting. Each bar represents the mean and standard deviation from three experiments.