Fig. 5. Combination of high-affinity human mAbs to distinct AM epitopes can detect low levels of LAM and AM in urine by sandwich ELISA.

Detection of serial dilutions of LAM and AM generated from the clinical Mtb strain CDC1551 and spiked into urine of a healthy volunteer by a L1AM04 (10 µg/mL) as a capture and T1AM09 (250 ng/mL) as a detection mAb, and b murine mAb CS-35 (10 µg/mL) as a capture and human mAb A194 (250 ng/mL) as a reference. The data shown are representative from two separate experiments.