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. 2021 Sep 29;12:680903. doi: 10.3389/fphar.2021.680903

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Copyright © 2021 Jiang, Huang, Zhang, Lv, Wang, He and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of pretreatment with K252a on the number of DCX-positive cells (Rb1-induced) in CSDS. (A) Representative immunofluorescence images of the dentate gyrus (DG) and costaining of the neuronal nuclear protein (NeuN) and DCX in green color. The scale bar is 200 and 20 μm for the representative and the enlarged images, accordingly. (B) The number of DCX + cells in the DG. (C–D) Represents immunoblotting of DCX coincided with immunohistochemical changes. N = 3–4 per group; the obtained data have been depicted as mean ± S.E.M. ^# p < 0.05, ^## p < 0.01, vs. the control group; ^* p < 0.05, **p < 0.01, vs. the model group.