Fig. 9.

Exosomes treatment reduces inflammation and regulates TrkC alternative splicing in microglia.

Immortalized microglial cells were treated with either (A) 10 ng LPS for 5 h or (B) 100 μM H₂O₂ for 1h followed by overnight (18 h) with either 2 μg hASC exosomes (H₂O₂ +EXO) or 2 μg MALAT1 depleted exosomes (H₂O₂ +EXO-M). Total RNA was isolated from control, H₂O₂, H₂O₂ treated with hASC exosomes (H₂O₂+EXO) or H₂O₂ treated with MALAT1 depleted hASC exosomes (H₂O₂+EXO-M) LPS, LPS treated with hASC exosomes (LPS+EXO) or LPS treated with MALAT1 depleted hASC exosomes (LPS+EXO-M). The experiment was repeated four times independently with similar results. SYBR Green qPCR was performed in triplicate with primers specific for TrkC_FL, TrkC_T1 or IL1β and β-actin as internal control. The graph shows relative quantification (RQ) with control set as reference. Percent exon 14 inclusion is calculated as (FL/(FL+T1))*100. Statistical analysis was performed using Sidak’s multiple comparisons test; ***p < 0.001 extremely significant, **p < 0.01 highly significant, *p < 0.05 significant, ns = not significant.