FIGURE 5.

Removal of tag from TrMgTx. (A) 16% Tricine–SDS-PAGE analysis of the TrMgTx samples incubated overnight at 25°C without (lane 1) and with (lane 2) factor Xa protease (at 1:200 enzyme to peptide ratio); M: low-molecular-weight protein marker. (B) RP-HPLC chromatogram shows the purification of UrMgTx. After separating the His-tag fragments with Ni⁺ beads, the digested sample was loaded on C₁₈ column and eluted with a gradient of 10–30% acetonitrile over 25 min. The absorbance was measured at 280 nm (left axis), dotted line shows the acetonitrile gradient (right axis). (C) ESI-QTOF-MS spectrum illustrates the average mass (4178.95 Da) of the purified UrMgTx.