TABLE 1.

TrMgTx purification scheme.

	Purification Step	Avg. Vol. (ml)	Avg. TrMgTx Concentration (mg/ml)	Avg. Total TrMgTx Amount (mg)	a Avg. Net Recovery (%)	Purity (%)
–	Cultured supernatant	246	0.085 b	20.8	100	–
1	Ni2+ affinity chromatography	14.5	1.01 b	14.7	70.6	–
2	RP-HPLC–	32.6	0.279 c	9.1	43.2	>98 d

^aNet recovery = TrMgTx acquired after a given step/total TrMgTx in cultured supernatant.

^bGel scan analysis with Image Lab Software (see Figure 2 legend).

^cPierce^TM BCA Protein Quantification kit.

^d% Purity was assessed by RP-HPLC (see Figure 4B).