Figure 2.

Generation and characterization of myeloid-specific Pfkfb3-deficient mice. (A) Diagram of the generation of myeloid-specific Pfkfb3-deficient mice (Pfkfb3^ΔMϕ) by crossing Pfkfb3^flox/flox mice with Lysm-Cre mice. (B) Representative genotyping PCR results of Pfkfb3^WT mice and Pfkfb3^ΔMϕ mice. (C) Representative Western blot results of Pfkfb3 and Actb in bone marrow (BM) and main organs of Pfkfb3^WT and Pfkfb3^ΔMϕ mice. (D) qPCR analysis of Pfkfb3 expression in BMDMs of Pfkfb3^WT mice and Pfkfb3^ΔMϕ mice (n = 5). (E) Representative Western blot results of Pfkfb3 and Actb in BMDMs of Pfkfb3^WT mice and Pfkfb3^ΔMϕ mice (top) and relative ratio of Pfkfb3/Actb (bottom) were quantitated by densitometric analysis of the corresponding Western blots (n = 5). (F) Representative images (left) of triple immunofluorescence for Pfkfb3 (red), Adgre1 (green) and nuclei (DAPI, blue), and quantification (right) of Pfkfb3 expression in BMDMs of Pfkfb3^WT mice and Pfkfb3^ΔMϕ mice (n = 5). All data are represented as mean ± SEM, ***P < 0.001 for Pfkfb3^WT vs. Pfkfb3^ΔMϕ (unpaired two-tailed Student's t test).