Figure 7.

Pfkfb3 inactivation in macrophages suppresses LPS-induced inflammation via the NF-κB signaling pathway. (A–E) Representative Western blot results (A) of phosphorylated and total level of Erk, Jnk, p38 and p65 in Pfkfb3^WT and Pfkfb3^ΔMϕ BMDMs treated with LPS (100 ng/mL) at the indicated time points, and relative ratio of p-Erk/Erk (B), p-Jnk/Jnk (C), p-p38/p38 (D) and p-p65/p65 (E) were quantitated by densitometric analysis of the corresponding Western blots (n = 4). (F,G) qPCR analysis of the mRNA levels of Il1b (F) and Il6 (G) in BMDMs treated with LPS (100 ng/mL) for 4 h, with or without AZ26 (10 μM) pretreatment for 30 min (n = 4). (H) Representative Western blot results (left) of p-p65, p65 and Actb in BMDMs treated with LPS (100 ng/mL) for 5 min, with or without AZ26 (10 μM) pretreatment for 30 min, and relative ratio of p-p65/p65 (right) was quantitated by densitometric analysis of the corresponding Western blots (n = 4). All data are represented as mean ± SEM, **P < 0.01 and *** P < 0.001 for indicated comparisons (unpaired two-tailed Student's t test).